PURPOSE:: To compute retinal vascular bed area (RVBA) in mm on distortion corrected ultra-wide field (UWF) fluorescein angiography (FA) images in eyes with retinal vein occlusion (RVO). METHODS:: Prospective, observational study. The peripheral distortion of baseline UWF FA (Optos 200Tx) images of 30 patients with RVO from the WAVE study (NCT01710839) and 13 control eyes of normal subjects was corrected using the stereographic projection method to compute RVBA in mm. RESULTS:: In comparison with age- and sex-matched normal controls, eyes with RVO had a decreased global RVBA for the entire retina (50.5 ± 20.4 mm vs 62.6 ± 12.2 mm, P = 0.023). Eyes with RVO and the un-affected fellow eye had a similar RVBA globally (50.5 ± 20.4 mm vs 46.2 ± 18.9 mm, P = 0.523). The RVBA was observed to negatively correlate with non-perfusion area (R = -0.47, P = 0.009). However, RVBA was not related to the severity of macular edema (ME) (P > 0.05). CONCLUSION:: Eyes with RVO have a similar RVBA to the un-affected fellow eyes but with a reduction when compared to normal controls. RVBA appears to be a surrogate biomarker of retinal ischemia on UWF FA but not the extent of ME.