Copper (Cu) and manganese (Mn) ions influenced laccase (Lac) and peroxidase production in Pleurotus eryngii, Pleurotus ostreatus, and Pleurotus pulmonarius. In P. eryngii, the optimum Cu concentration for Lac production was 1 mM and for peroxidases 10 mM, and Mn concentration of 5 mM led to peaks of Lac and peroxidase activity. In P. ostreatus HAI 493, the highest level of Lac activity was at Cu concentrations of 1 and 10 mM and Mn concentration of 1 mM, respectively. The absence of Cu and Mn caused the highest levels of peroxidase production. In P. ostreatus HAI 494, the highest level of Lac activity was at a Cu concentration of 5 mM and at Mn concentration of 1 mM, respectively. High levels of peroxidase activity were found in the medium without and with 1 mM Cu, and at 1 and 5 mM Mn, respectively. In P. pulmonarius, the highest Lac activity was found in the presence of 5 mM Cu and 5 mM Mn, respectively. The absence of Cu and Mn as well as their presence at a concentration of 1 mM led to the peaks of peroxidase activities.