The purpose of this study is to analyze the impact of the organizational culture of local public enterprises on job satisfaction, and in particular to verify the mediating effect of trust. For this purpose, organizational culture is divided into developmental, rational, consensus, and hierarchical cultures, job satisfaction is divided into environmental, reward, and psychological satisfaction, and trust is divided into supervisor, organization, and coworker trust. The data used was a survey of 357 people working at local public enterprises under Gwangju Metropolitan City. The analysis showed that among organizational cultures, developmental culture and consensus culture have a positive effect on job satisfaction, while hierarchical culture has a positive effect only on psychological satisfaction. Developmental culture, consensus culture, and hierarchy culture have a positive impact on trust, and trust factors have a positive impact on job satisfaction. Trust was found to partially mediate the relationship between organizational culture and job satisfaction. In conclusion, in order to improve employee job satisfaction in local public enterprises, it is necessary to activate communication and collaboration with employees, delegate autonomy and authority in decision-making, make efforts to improve employees' capabilities, and expand welfare systems. It is also necessary to improve work processes, improve the working environment, and strengthen cooperation among employees. In order to improve trust as a mediating variable, it was suggested that decision-making process and transparent information disclosure, establishment of a fair work evaluation system, strengthening communication between supervisors and subordinates, enhancement of supervisors' work capabilities, and respect and consideration for subordinates' opinions were necessary.