Abstract Objectives To assess the absorption of a-tocopherol acetate and 18b-glycyrrhetinic acid, which are used as active ingredients in toothpaste, into a reconstructed gingival tissue. Methods EpiGingival TM tissues were treated with a 25% slurry of toothpaste containing 2% a-tocopherol acetate and 0.3% 18b-glycyrrhetinic acid, for 2 minutes. The treatment was repeated up to 6 times, with 1 hour intervals. After completion of all treatments, the active ingredients in the tissue extracts and receiver solutions were measured by high performance liquid chromatography. Results Although a-tocopherol acetate was not detected, α-tocopherol was detected in the tissue extracts, indicating that a-tocopherol acetate was bioconverted to α-tocopherol after absorption. We could detect 18β-glycyrrhetinic acid both in the tissue extracts and in the receiver solutions, with a positive correlation to the number of treatments. Conclusions We found that our toothpaste effectively delivered α-tocopherol acetate and 18α-glycyrrhetinic acid to a reconstructed gingival tissue in vitro .