Background The tracheal bronchus in Kartagener syndrome (KS) is a rare case that may cause difficulty in one-lung ventilation (OLV). Here we reported a case of successful OLV using bronchial blocker in a patient with tracheal bronchus and KS. Case A 66-year-old female patient with KS was admitted for left-side diaphragmatic plication. The patient’s preoperative computed tomography image showed a tracheal bronchus of the apical segment in the right upper lobe. The patient received epidural analgesia and general anesthesia through total intravenous anesthesia. An EZ-Blocker ® (Teleflex Life Sciences Ltd., Ireland) was used to perform OLV. Conclusions OLV through an EZ-Blocker ® can be successfully performed in tracheal bronchus patients with KS without side effects.