Effect of bFGF on the MCF-7 Cell Cycle with CD44+/CD24-: Promoting the G0/G1→G2/S Transition
- Resource Type
- Text
- Authors
- Han, Zhao-dong; Yang, Zhen-lin; Cheng, Kai
- Source
- Journal of Breast Cancer. 15(4):388-392
- Subject
- Original Article
- Language
Purpose Few cells with stem cell characteristics possess capabilities of self-renewal and differentiation, which leads to high tumorigenesis and resistance to standard chemotherapeutic agents. These cells are mostly quiescent, and arrest occurs at the mitotic G0/G1 phase in mitosis. We explored the effects of basic fibroblast growth factor (bFGF) on the MCF-7 cell cycle with CD44 + /CD24 - . Methods Cancer-initiating cells were propagated as mammospheres. The CD44 + /CD24 - subpopulation was sorted by a fluorescence activating cell sorter-Vantage flow cytometer. A cell cycle analysis was performed with different bFGF concentrations. Results Differences in the CD44 + /CD24 - cell proliferation under different bFGF concentrations were observed ( p =0.001). When the bFGF concentration was increased, the proportion of CD44 + /CD24 - at G0/G1 decreased ( p =0.023). Conclusion We conclude that bFGF may sustain CD44 + /CD24 - cell proliferation and could promote cell progression through the G0/G1→G2/S phase transition.