Purpose: To provide a real-world comparison of the up-to-date, digitally assisted vitreoretinal surgery systems (DAVSs) ARTEVO 800 and NGENUITY. Methods: We reviewed the medical records of patients who underwent cataract surgery, vitrectomy, or phacovitrectomy using either the ARTEVO 800 or the NGENUITY system. Operating times and intraoperative complications along with the manufacturer’s specifications and surgical images were collected for both systems. Results: Three-hundred four eyes of 296 patients were recruited. The difference in mean operating time between the ARTEVO 800 (n = 29) and the NGENUITY (n = 267) was not significant for any surgical procedure. The only intraoperative complication was posterior capsular rupture with the ARTEVO 800 during cataract surgery. There was also no significant difference in the surgical parameters or manufacturer specifications. However, the ARTEVO 800 and the NGENUITY created different surgical images. The ARTEVO 800 provided a higher resolution, greater light sensitivity, and a wider field of view than the NGENUITY, but it also produced saturation artifacts. The NGENUITY had a high dynamic range without any saturation artifacts and more detailed settings for adjusting the surgical image. Conclusions: No obvious difference was found in operating time, intraoperative complications, or manufacturer specifications between the two systems. However, different surgical images were present in a real-world environment, and they could have influenced the actual surgical performances in several situations. Our findings provide insights and practical tips for real-world application of DAVSs.