Background: Stress assessment is a crucial aspect of mental health monitoring. The Perceived Stress Scale (PSS), a self-report inventory, assesses the level of stress individuals perceive in their daily lives. This study aimed to evaluate the reliability and validity of PSS shared via a mobile application. Methods: The study included 1,327 residents. All participants completed the PSS, the nine-item Patient Health Questionnaire, and the seven-item Generalized Anxiety Disorder (GAD-7) scale. Results: The average Cronbach’s alpha value for the PSS was 0.938, which is satisfactory. PSS (r=0.631, p<0.01) and GAD-7 (r=0.622, p<0.01) exhibited significant positive correlations. Factor analysis yielded two factors with eigenvalues of 7.855 and 1.463, accounting for 66.56% of the variance. Factor 1 represented “positive” while Factor 2 denoted “negative.” Conclusion: The PSS is an appropriate measurement tool for perceived stress level and maintains its reliability and validity when administered through a tablet PC or smartphone application. Using a mobile application to evaluate PSS in the community would be beneficial.