Purpose: The purpose of this study was to investigate factors influencing care workers’ intention of hand hygiene implementation in long-term care hospitals. Methods: A total of 180 care workers working at long-term care hospitals were recruited. Data collection was done from July 22 to September 7, 2018. Results: The significant TPB variables influencing the intention of hand hygiene implementation were perceived behavior control (β=.41, p<.001), normative belief (β=.28, p<.001) and attitude toward behavior (β=.15, p=.014). These factors explain 39% of care workers’ intension of implementing hand hygiene in long-term care hospitals. Conclusion: In order to strengthen the commitment of hand hygiene, it is necessary to have a positive attitude toward hand hygiene by eliminating the obstacles to hand hygiene.