Non-human leukocyte antigen antibodies (N-HLA Abs) have been suggested to impact of with kidney allograft rejection and graft loss in kidney transplantation (KT). We report three KT cases of patients with N-HLA autoAbs in antibody-mediated re-jection (AMR) and T cell-mediated rejection (TCMR) showing negative panel-reactive antibody (PRA), donor-specific antibody DSA), and C1q antibody (C1qA). First case was a 62-year-old male who underwent graft nephrectomy 12 days after first KT due to AMR. Using LABScreen Autoantibody assay (One lambda), nine of 39 N-HLA autoAbs were (vimentin, PRKCH, CXCL11, CXCL10, GAPDH, ARHGDIB, HNRNPK, IFNG, and REG3A) were above a cut-off of 95% nonsensitized population (NSP). The sec-ond case, a 42-year-old male, presented with both AMR and acute TCMR at 16 months 16 days after KT. None of 39 N-HLA au-toAbs were above a cut-off of 95% NSP, but three non-HLA autoAbs (FLRT2, AGRIN, and GSTT1) were above of cut-off of 85% of NSP. He was discharged after therapeutic plasmapheresis, rituximab treatment, steroid pulse therapy (SPT), and antithymocyte globulin rescue therapy. The third case, a 48-year-old male, presented with third acute TCMR at 33 months 7 days after first KT. In three N-HLA autoAbs (FLRT2, CXCL10, and CXCL9) above a cut-off of 85% NSP, especially CXCL9 was above a cut-off of 95% NSP. He discharged after SPT. Although N-HLA autoAbs were heterogeneous regarding the target antigens, positive cut-offs, and mean fluorescence intensity in this study, they might be associated with TCMR as well as AMR. To clarify the role of N-HLA autoAbs in KT, further evaluation on cut-offs for positivity using large population is needed.