Background The aim of this study was to investigate the association between quality of life questionnaire at diagnosis and survival of lung cancer. Methods This was a multicenter retrospective study from lung cancer patients responded to the quality of life questionnaire using EORTC QLQ-C30 v.3.0 between December 2017 and December 2020. We analyzed 5 functional (physical, role, emotional, cognitive, social functioning) and 9 symptom (fatigue, nausea and vomiting, pain, dyspnea, insomnia, appetite loss, constipation, diarrhea, financial difficulties) scales and examined their associations with survival. Results A total of 1297 lung cancer patients were enrolled (adenocarcinoma [55%], squamous cell carcinoma [25%] and small cell carcinoma [8%]). The mean age was 68.2, and the majority were male (69%). 512 (39.5%) patients were stage I or II, and 60.5% of those was stage III or IV. On multivariate analysis, female (p=0.013), younger age (p<0.001), never-smoker (p=0.007), stage I or II (p=0.004 and 0.026, respectively), higher physical functioning (PF2) (p=0.034) and emotional functioning (EF) (p=0.021), were favorable independent predictors for survival, significantly. On subgroup analysis according to early (stage I and II) or advanced (stage III or IV) stage, higher PF2 and EF scales were also independent prognostic factors for favorable outcome, respectively. Among symptom scales, fatigue, pain, insomnia and financial difficulties were poor risk factors for EF scales. Fatigue, pain, dyspnea and financial difficulties were significantly associated with low PF2 scales. Conclusions The present study showed higher PF2 and EF scale among quality of life questionnaire items measured by EORTC QLQ-C30 were independently associated with better survival in lung cancer regardless of clinical stages. In addition, fatigue, pain and financial difficulties were associated with lower EF and PF2 among symptom scales.