Generation of Newly Discovered Resistance Gene mcr-1 Knockout in Escherichia coli Using the CRISPR/Cas9 System
- Resource Type
- Article
- Authors
- Lichang Sun; Tao He; Lili Zhang; Maoda Pang; Qiaoyan Zhang; Yan Zhou; Hongduo Bao; Ran Wang
- Source
- Journal of Microbiology and Biotechnology. Jul 31, 2017 27(7):1276
- Subject
- mcr-1
knockout
E. coli
CRISPR/Cas9 system
BMAP-27
- Language
- Korean
English
- ISSN
- 1017-7825
The mcr-1 gene is a new “superbug” gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas:mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.