PCR을 이용한 salmonella enteritidis의 특이적 검출
Specific detection of salmonella enteritidis using polymerase chain reaction method
- Resource Type
- Article
- Authors
- 이정학 / Jung Hak Lee; 이병동 / Byung Dong Lee; 조미영 / Mi Yeong Jo; 여용구 / Yong Gu Yeo; 김영섭 / Young Seb Kim
- Source
- 한국동물위생학회지 (KOJVS) / Korean Journal of Veterinary Service. Sep 30, 2000 23(3):227
- Subject
- Salmonella species
Polymerase chain reaction
PCR
Electrophoresis
- Language
- Korean
- ISSN
- 3022-7372
Salmonella enteritidis is the most prevalent etiologic agents of foodhome acute gastroenteritis. Direct isolation and identification of S enteritidis are time consuming work and not so highly sensitive. This study was conducted to develop for the specific detection of S enteritidis using polymerase chain reaction(PCR). PCR primers were selected to amplify a 351 -base pair(bp) DNA fragment from the salmonella plasmid virulence A(spv A) gene of S enteritidis. With the primers, 351 bp DNA products were amplified from S enteritidis but not from other B, D, Cl serogroup Saimoneilci spp. It was sensitive to detect up to 40 pg of template DNA by agarose gel electrophoresis. This PCR assay is very rapid and specific method and less time consuming than the standard bacteriological methods.