Medical biochemistry, which studies life dynamics at the material level, is a very important discipline for the advancement of newest medical knowledge. Metabolism is the sum of chemical reactions and energy conversions in an organism that are essential for the maintenance of life and has reaction networks of diverse molecules and complex control mechanisms. These microscopic and intricate characteristics require efforts for long periods of time and a high level of cognitive ability for the learning of biochemistry and make the achievement of the educational goals of biochemistry focused on the understanding of core concepts and interconnectivity a very burdensome challenge to not only medical college students but also to teachers. In addition, unidirectional and conservative forms of lectures and textbooks full of rigid diagrams have been factors that make students more dependent on simple memorization.Empathizing with such difficulties experienced by medical college students in their learning of biochemistry, this study aimed to help learners easily learn the difficult information of biochemistry through VR visualization, and to help them cultivate their scientific reasoning ability. To this end, this study was conducted with three goals; (1) construction of 'VR Visualization Model' related to information and spatiotemporal, (2) theoretical validation of the 'VR Visualization Model', and (3) application of the 'VR Visualization Model' to the learning of biochemistry and the evaluation of the memory effects.1) Construction of 'VR Visualization Model' related to information and spatiotemporalThe VR visualization model is designed to provide the VR authoring environment necessary for the visualization of biochemical learning topics, and consists of a VR system and a space design process. First, the VR system consists of a user input interface for interactions with the HMD, which is a display device capable of expressing 3D space, and computer hardware and software for VR authoring and operation, and graphic design. In order to construct an optimal VR system, multifaceted performance and compatibility tests were conducted. Second, through a review of literature related to human spatial cognition, the theoretical foundation and design process necessary for the method of spatial composition of VR visualization were established. 2) Theoretical validation of the 'VR Visualization Model'Whether the VR visualization model appropriately gives spatiotemporality in visualizing information and how the linkage between information and space-time helps learning and memory improvement were verified based on related theories. First, to identify in what form the VR visualization model provides spatiotemporality to information per se and users, the attributes of VR defined in diverse pieces of literature were examined. In addition, the chronophotographic approach was introduced as a method to identify and evaluate the spatiotemporality of human activities and human activities were simulated and visualized on the ‘Time Geography’ coordinate system to quantify the spatiotemporality of human activities. Second, with regard to the issue of the relationship between the spatiotemporality of information given through VR and memory, a theoretical framework was established based on brain and neural science literature and the validity of the VR visualization model was verified. 3) Application of the 'VR Visualization Model' to the learning of biochemistry and the evaluation of the memory effectsTo evaluate the effects of the VR visualization model, the VR visualization model was applied to the TCA cycle, which is felt the most burdensome by students. The experimental group used the VR visualization model while the control group used PowerPoint displays using 2D diagrams to learn the TCA cycle and tests were conducted. The results of two times of evaluation of short - term memory and long-term memory indicated that learning using VR visualization was more effective than learning using teaching materials containing 2D planes.In the test of short-term memory, learning using VR visualization exceptionally did not show any statistically significant effect on general category items, and was more effective on the memory of more complicated sub-category and sub-sub category items.In the test of long-term memory conducted one week later, it was verified that the learning using VR visualization was more effective in all the general category items, sub-category, and sub-sub category items. These results can be said to support the assumption that the VR visualization model can be helpful in learning and remembering complicated biochemical information.Recent information technology innovations have brought many changes to the educational environment and thanks to the technological development, science visualization has become the focus of the educational reform for the formation of a new way of thinking that combines the elements of modern education; intuition, reasoning, and imagination. The VR visualization in this study is using time and space as a framework for learning and memory to provide structured information to space and compress various elements of experience into one coherent interaction. The implementation of such tasks requires the integration of multidisciplinary knowledge and resources, such as information technology and medical and scientific understanding of design and cognition. To this end, a VR visualization model consisting of a VR computer system and a space design process was constructed, a conceptual model, which is a theoretical foundation, was established to ensure the validity of the study, and the utility of the VR visualization model for biochemical learning was evaluated. This study attempted to prepare a system and theoretical basis for visualization of VR for education, for which integrated research has been insufficient thus far. Through the attempt, this study presented results proving that VR visualization can have positive effects on learning and memory of complex tasks such as biochemistry.To expand this study into solutions for future scientific and educational issues, updated theories of brain and neural science such as spatial cognition should be continuously applied to strengthen the concept, logic, and physical models of visualization. In addition, this researcher expects that studies will be expanded not only to the improvement of memory but also to the relationships between pieces of information in medical biochemistry to be able to provide learners with medical insights to human life.