As aging society has become a serious issue all over the world, researches have been advanced day by day on implantable medical devices to replace or assist aging organs. From the perspective of medical care engineering, the biocompatibility of inserted material is an important issue for the implantable medical device. However, when a device to be inserted into the human body is composed of a commonly used material such as polymer, an immune response such as an inflammatory reaction cannot be avoided due to the harmfulness of the starting compound and the complicated manufacturing process. The medical field is focusing on natural materials, such as human tissue derived from patients that are biocompatible and human-friendly to overcome the limitations of material selectivity. Still, additional surgery is required to obtain natural material derived from the human body, which places a great burden on the human body. In order to avoid such further operation, human tissue collected from the cadaver is sometimes produced through radiation or chemical treatment, but it is unethical and since this is also an externally derived material, an immune response cannot be avoided. To obtain the human-friendly material in ethical method, we focus on the iPSC (induced pluripotent stem cell) differentiation. iPSC is a type of stem cell obtained by differentiation from a patient's skin cells. Therefore, by using iPSC, it is possible to provide a material tailored to each patient. Tissue engineering is essential for culturing cells in vitro. However, existing tissue engineering is not suitable for subjecting the resulting cell sheet to further operation. We propose oxygen terminated graphene-based time dilation scaffold cell culture platform suitable for cell sheet fabrication and conducted their characteristic evaluation. We conduct pre-study using C2C12 mouse myoblast cell line for conformity determination on oxygen terminated graphene. ANNEXIN V/Pi staining was performed to verify the toxicity of oxygen terminated graphene to cell culture. And also, to differentiation efficiency verification of oxygen terminated graphene used to provide a cell culture platform, qRT-PCR, western blot is conducted.