This paper studies the application of eggshell membrane. Eggshell membrane is a good source of protein, but it can not dissolve in solvent, limit the development of eggshell membrane, make eggshell membrane become soluble eggshell membrane Protein, and obtain soluble protein nanofiber by electrospinning technology. Electrospinning technology gives the fabric a rich porosity and a large surface area, ensuring enhanced water absorption and cellular respiration. In order to improve the processability of the eggshell membrane, it is mixed with chitosan to enhance physical properties. Electron microscopy (SEM) images of thermal properties have been considered to characterize fabric properties, and Triboelectric performance tests have been used to characterize the response of fabrics to different materials. Soluble eggshell membrane for 3D printing, mixed with PLA, using FDM technology. Water contact angle and mechanical properties are improved compared to pure eggshell membranes. Soluble eggshell membrane proteins were added to the gel for bio 3d printing. Improve print performance and improve performance, such as the compression performance of printed products.