The overlapping cuticle scales on the wool surface cause severe felting shrinkage during laundering. However, theconventional wool anti-felting processing mostly adopts dichloroisocyanurate (DCCA), which produces absorbable organichalogen (AOX) with high toxicity in the effluents and cause severe environmental problems. Herein, an environmentalfriendlyenzymatic method was proposed and investigated to endow satisfactory shrink-proofing property for wool fabricswithout severe damages. The cutinase from Thermobifida fusca and the keratinolytic enzyme from Bacillus subtilis wereconsecutively used to treat wool fabrics for 4 h and 24 h, respectively. The area shrinkage of the resultant wool fabricsdecreased from 12.4 % to 5.86 % for to the cuticle was partly broken, which met the machine-washable requirement with anacceptable strength loss of 7.46 %. Moreover, the dyeability and wettability of the resultant fabric were improved after thecombined enzymatic treatments, due to the destruction of the lipid layer and breakdown of keratin by combined cutinase andkeratinolytic enzyme according to the analyses such as scanning electron microscope (SEM) and X-ray photoelectronspectroscopy (XPS) and contact angle. Overall, our result revealed that the synergistic actions of cutinase and keratinolyticenzyme treatments could effectively disintegrate cuticles and remove scales.