Novel efficient biochar of Goldenberry peels (GBPMW-H3PO4) was prepared through a microwave-assistedphosphoric acid activation method. It was characterized and used for removing two beta-lactamase inhibitors, sulbactam(SAM, first listed in Japan in 1986) and avibactam (AVI, first listed in the U.S. in 2015), from aqueous solution. Characterization confirmed that GBPMW-H3PO4 displayed a high surface area (720.046m2 g1), more abundant porestructure, smaller particle size, and higher thermal stability. The experimental results showed that the adsorption of thetwo antibiotics was a spontaneous, favorable, and endothermic process, highly dependent on solution pH. A contacttime of 60 min assured equilibrium, and GBPMW-H3PO4 followed pseudo-first-order kinetics (R2=0.9950-0.9977). Furthermore,the adsorption capacities of GBPMW-H3PO4 for SAM and AVI were 211.86 and 198.81mg g1, respectively,and the performance was better than that of unmodified biochar. Microscopically, the main mechanism could beexplained by - electron donor-acceptor interaction, hydrogen bonding interaction, -hydrogen bonding, hydrophobicinteraction, and electrostatic interaction. The study demonstrates that the microwave-assisted H3PO4 activationmethod could produce biochar, and GBPMW-H3PO4 was confirmed to be a low-cost and high-efficiency adsorbent forremoving beta-lactamase inhibitors from medical wastewater.