A New Rapid Spectrophotometric Quantitative Determination Method for γ-Decalactone and Application in High-throughput Screening for γ-Decalactone Producing Strains
- Resource Type
- Article
- Authors
- Yuping Zhao; Xiaoqing Mu; Yao Nie; Yan Xu
- Source
- Food Science and Biotechnology, 23(6), pp.1935-1940 Dec, 2014
- Subject
- 식품과학
- Language
- English
- ISSN
- 2092-6456
1226-7708
A new, rapid spectrophotometric quantitativedetermination method for γ-decalactone (GDL) was developedbased on the ferric hydroxamate reaction. Samples addedto a mixture of NH2OH·HCl and NaOH were heated toreact. Then HCl was added. Ferric chloride was added forcoloration, and 75% (v/v) ethanol was added. Absorbancevalues were measured after coloring for 10 min. Theconcentration range of a GDL calibration curve was 0.125-8.14 g/L. A 96-well plate high-throughput screening methodwas established to obtain desirable strains. A total of 215GDL-producing strains were identified from 4,327 samples. The highest production was 179 mg/L.