Isolation and Characterization of a Putative Vacuolar Na+/H+ Antiporter Gene from Zoysia japonica L.
- Resource Type
- Article
- Authors
- Yanhua Du; Qian Hei; Yaxin Liu; Hui Zhang; Kai Xu; Tao Xia
- Source
- Journal of Plant Biology(한국식물학회지), 52(4), pp.251-258 Aug, 2010
- Subject
- 생물학
- Language
- English
- ISSN
- 1226-9239
A Na+/H+ antiporter gene from Zoysia japonica (ZjNHX1) which is a member of plant NHX-genes family was cloned by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE)technology. The isolated cDNA is 2,421 bp in length and contains one open-reading frame (ORF) which comprises 540amino acid residues and shows higher homology with other plant Na+/H+ antiporters. ZjNHX1 could partially complement the salt-sensitive phenotypes of Δnhx1 and Δena1-4Δnhx1 yeast mutants in the presence of NaCl, KCl, and LiCl. The expression of ZjNHX1 in Z. japonica increased after NaCl treatment and this result accords with that of Na+contents determination under the same treatment. These results implied that ZjNHX1 functions as a vacuolar Na+/H+antiporter and plays an important role in salt tolerance and ion homeostasis in Z. japonica.