Soil salinity is the primary limiting factor affecting the growth and production of vegetable soybean[Glycine max (L.) Merrill] worldwide. Plants can survive in salinity conditions through the mechanism of sodium ion(Na+) compartmentalization, during which the vacuolar Na+/H+ antiporter (NHX) genes play a critical role. In the presentstudy, a novel vacuolar NHX gene from Solanum torvum Swartz ‘Torvum Vigor’ (StNHX1; GenBank accessionnumber: JN606860.1) was isolated and transferred into the vegetable soybean ‘Xinliaoxian’ through an Agrobacteriummediatedprotocol. The PCR and southern blot analyses confirmed the successful integration of the exogenous genes. The GUS assay and semi-quantitative RT-PCR analysis showed that the alien genes were inherited by the progeniesand that the StNHX1 gene was overexpressed in the T3 generation. The overexpression of this gene conferred hightolerance to salt stress. Under 100 mM NaCl conditions, the scorch scores and the Na+ and malondialdehyde (MDA)contents of the leaves of the transgenic lines were significantly lower as compared with the wild-type (WT) plants,while the K+/Na+ ratio, the contents of K+, relative chlorophyll and relative water, and some morphological traits of thetransgenic plants were significantly higher than those of the WT. These results indicate that the overexpression ofStNHX1 enhances the salt tolerance of vegetable soybean and that StNHX1 is one of the promising target genes thatcan be manipulated to improve the salinity tolerance of crops.