Bioleaching studies for chalcopyrite contained ball mill spillages are very scarce in the literature. We developeda process flow sheet for the recovery of copper metal from surface activated (600 oC, 15 min) ball mill spillagethrough bio-hydrometallurgical processing route. Bioleaching of the activated sample using a mixed meso-acidophilicbacterial consortium predominantly A. ferrooxidans strains was found to be effective at a lixiviant flow rate of1.5 L/h, enabling a maximum 72.36% copper recovery in 20 days. Mineralogical as well as morphological changesover the sample surface were seen to trigger the bioleaching efficiency of meso-acidophiles, thereby contributing towardsan enhanced copper recovery from the ball mill spillage. The bio-leach liquor containing 1.84 g/L Cu was purified throughsolvent extraction using LIX 84I in kerosene prior to the recovery of copper metal by electrowinning. Purity of thecopper produced through this process was 99.99%.