Antibacterial and antioxidant properties exert an indispensable effect on biomedical and packaging materialswhich are mainly made of fiber-based fabrics. The current research illustrates a novel type of composite fiber which wasprepared from thermoplastic polyurethane (PU), polyacrylonitrile (PAN) and tea polyphenols (TP) through wet-spinningtechnology. The PU-PAN mass ratio was optimized upon the mechanical property; TP was incorporated into the compositefiber with different contents. The obtained fibers were fully characterized in terms of mechanical, thermal, morphologicalproperties as well as surface wettability. Additionally, in vitro TP release, antioxidant and antibacterial activity of suchcomposite fibers before and after washing were under investigation systematically and orderly. The findings showed that TPloadedcomposite fibers with an average diameter around 120 μm performed a sustained TP release behavior and desirablecytocompatibility. According to the capacity on scavenging 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radicals, theobservation of scavenging ratio reaching to 97 % proved the excellent antioxidant performance. Furthermore, the idealantibacterial activity against Gram-negative E. coli and Gram-positive S. aureus was also demonstrated. Collectively, thisstudy provides a facile strategy to design and prepare a novel type of composite fibers and fabrics, which may be put intoapplication as wound dressing and tissue conduit.