An electrochemical sensor (Cu2+-IIPs/GCE) was developed for detection of Cu2+ in water. Cu2+-IIPs/GCE was prepared by dispersing Cu2+ imprinted polymers (Cu2+-IIPs) on a preprocessed glassy carbon electrode. Cu2+-IIPs were synthesized on the surface of modified carbon spheres by ion imprinting technology. The electrochemical performance of Cu2+-IIPs/GCE was evaluated by differential pulse voltammetry method. The response of Cu2+-IIPs/GCE to Cu2+ was linear in 1.0 x 10 -5 mol/L to 1.0 x 10 -3 mol/L. The detection limit was 5.99 x 10 -6 mol/L (S/N = 3). The current response value of Cu2+-IIPs/GCE was 2.14 times that of the nonimprinted electrode. These results suggest that Cu2+-IIPs/GCE can detect the concentration of Cu2+ in water, providing a new way for heavy metal ions adsorption and testing.