Phytochemical investigation of the ethanolextract of the roots of Sanguisorba officinalis resulted inthe isolation of three new triterpene glycosides, 3b-[(a-Larabinopyranosyl)oxy]-19a,23-dihydroxyolean-12-en-28-oicacid 28-[6-O-acetyl-b-D-glucopyranosyl] ester (1),2a,3b,19a,23-tetrahydroxyurs-12-en-28-oic acid 28-[6-Oacetyl-b-D-glucopyranosyl] ester (2), and 3b-[(a-L-arabinopyranosyl)oxy]-19a-hydroxyurs-12,20(30)-dien-28-oicacid 28-[6-O-acetyl-b-D-glucopyranosyl] ester (3). All thetriterpene glycosides exhibited the significant cytotoxicpotential with low IC50 values (IC50\5.0 lM) against sixtumor cell lines (MCF-7, HeLa, HepG2, SGC-7901, NCIH460,and BGC-823).