To improve the flotation recovery of tungsten minerals was of an urgent issue. In this paper, N-[(3-hydroxyamino)-propoxy]-N-hexyl dithiocarbamate (HAHD) was designed as a powerful collector toenrich and separate scheelite particles. In comparison to benzohydroxamic acid (BHA), HAHD dramaticallypromoted the floatability of the Pb(Ⅱ)-activated scheelite. Meanwhile, HAHD exhibited a competentselectivity for flotation separation of scheelite from calcite. The findings of zeta potential, contact angle,FTIR, UV and XPS deduced that both HAHD’s dithiocarbamate and hydroxamate groups were of the reactivecenters to the Pb(Ⅱ)-activated scheelite where the surface HAHD-Pb(Ⅱ) complex was generated. Theintroduction of the dithiocarbamate group had a limited influence on HAHD’s flotation affinity towardscalcite, while strengthened HAHD aggregation on to the Pb(Ⅱ)-activated scheelite surface, the floatabilitydifference between scheelite and calcite significantly increased. Moreover, the ‘‘h-shape” adsorptionmode and hexyl group of HAHD possessed a stronger hydrophobization towards scheelite than thesingle-site adsorption and benzene ring of BHA, returning a higher flotation recovery of scheeliteparticles.