The widespread addition of sulfa antibiotics in feeds has long been an environment safety issue. Therefore, it is quite meaningful to constantly propose better solutions for determining the sulfaantibiotics in the environment. Herein, molecularly imprinted electrospun nanofibre membrane (MIM)assisted stir bar sorptive extraction method for the determination sulfonamides (SAs) in feed wasdeveloped. In this study, molecularly imprinted polymers (MIPs) werefirstly prepared via emulsionpolymerization using sulfamonomethoxine as template. Then, the morphology-controllable MIM wasobtained with MIPs-doping electro-spinning solution using electro-spinning technique. Next, MIM arecoated on the magnetic stir bar by heat-sealing. Finally, MIM coated stir bar was used for selectiveenrichment of SAs. The obtained MIM exhibited excellent class selectivity towards SAs (the selectivityfactor β is 2.3–2.7). The MIM coated stir bar can be directly applied to extract SAs from feed samples. Under the optimum extraction conditions, the detection limit of the method could reach 1.5 3.4 ng/g. And satisfactory recoveries ranging from 80 6% to 89 7% were achieved under all three spiked levelsfor determination of four SAs infive different feed samples. The present work not only offers insights todevelop magnetic stir bar using imprinted membrane, but also provides a new method for SAs extraction.