Objective: This study aims to investigate the feasibility of using optical illusions for safety and health to address the high accident rates of falls and collisions in industrial settings worldwide. Background: A Study on the Application of Optical Illusion Safety Signs in Industrial sites – Focusing on Fall and Collision Prevention. In many cities worldwide, efforts are being made to prevent accidents by creating an optical illusion on crosswalks that gives approaching vehicle drivers the perception that the crosswalk is floating above the road. Method: For this study, five optical illusions were selected through focus group interviews (FGI), and a survey was conducted among 367 participants, including 48 safety managers and 319 workers. The survey was conducted using a 5-point Likert scale, and participants were asked to select words that best described their perception of each illusion. Cronbach's Alpha and ANOVA were conducted as part of the study. Results: The results showed that Cronbach's Alpha value for the questionnaire was above 0.8, indicating good internal consistency. Concerning falls, Optical Illusion 2 was significantly rated higher than Optical Illusion 1. Optical Illusion 5 was significantly rated higher than Optical Illusion 3 and 4 in the collisions. In summary, Optical Illusion 2 and 5 were found to be highly applicable in the real-world settings. Conclusion: These findings suggest that safety signs utilizing optical illusions are likely to be highly effective in preventing accidents. Application: However, for these safety signs to be effective in accident prevention, additional consideration should be given to user learning effects, distraction effects, and the psychological impact on users.