Background Bisphenol A (BPA) and its alternatives (BPF, BPS) are widely used in daily consumer products, which commonly expose humans to these chemicals. Bisphenols (BPs) are endocrine-disrupting chemicals widely present in the environment and can accumulate in the entire food chain by circulating in the ecosystem. In humans, BPs intake leads to accumulation in the human body and causes many chronic diseases, including endocrine disruption, immunotoxicity, and cancer. Objective Although several studies have demonstrated the negative eff ect of BPA and its alternatives, studies have yet to be determined whether bisphenols can aff ect human trophoblast cells. Thus, this study aimed to analyze the toxic eff ect of BPs on the human trophoblast cell line BeWo. Results We investigated the cytotoxic eff ect of BPs on human trophoblast cells. The cytotoxicity was measured by the MTS assay and fl ow cytometric analysis that representing the cell viability, cell proliferation, cell cycle progression and apoptotic cell death. BPs had no eff ect on BeWo cell viability at concentrations below 100 mM, and did not cause signifi cant changes in cell proliferation and apoptosis. However, BPA and BPS altered cell cycle progression and checkpoint regulator expression. Conclusion BPs did not aff ect cell viability and proliferation. However, BPS altered the G 0 /G 1 and G 2 /M phases of the trophoblast BeWo cells by increasing cyclin B1 expression.