Phoma wasabiae was cultivated on the potato culture solution containing 2% glucose at 25℃. The color of the culture solution changed gradually to reddish orange at the surface of the medium with growth of the mold. After three weeks of the cultivation, the color of the medium turned blackish. The blackish culture solution was concentrated in vacuo. The concentrated solution was extracted with chloroform and the solvent was removed. Five pigments were isolated from the resulting oily substance (0.24 g/l) by preparative silica gel TLC using benzene-methanol (9:1) as eluent (R_f values: 0.45, 0.33, 0.26, 0.19, and 0.12) and named wasabidienone-A, B, C, D, and E, respectively. The structures of four pigments, WA, WB, WD, and WE, were elucidated as 1, 8, 12, and 16, respectively, having a novel cyclohexadienone moiety from spectral and chemical evidences. The structural elucidation of WC is now under investigation.