An exogenous neurite outgrowth factor, Z-LLL-H, induces neurite outgrowth of PC12 cells as progenitor cells of peripheral nervous system. In the present study, we examined the effects of Z-LLL-H on the neural stem cells derived from pluripotent embryonic stem cells. Z-LLL-H induced the neurite outgrowth of neural stem cells in a concentration-dependent manner as well as PC12 cells, but had a little effect on the cell-viability at a lower concentration than 40nM. Furthermore, both of immunohistchemical and RT-PCR analyses confirmed that neural stem cells by the addition of Z-LLL-H resulted in some kinds of the neurite-extended and differentiated neurons. We discussed also the usefulness and possibility to apply these neurons induced from embryonic stem cell-derived neural stem cells to regeneration of nervous system diseases.