This study was carried out to examine the growth characteristics of anthocyanin accumulative and non-accumulative strains which were separated from creeping bentgrass (Agrostis stolonifera L.) cultivar “Penncross”. Both strains were selected from Penncross green of 6 years after establishment and propagated in nursery greens. To compare growth characteristics of these strains, plants were grown in field and pot conditions. Growth of anthocyanin accumulative strain was low in winter and vigorous toward summer. While, anthocyanin non-accumulative strain had lower growth rate than anthocyanin accumulative strain in summer. Anthocyanin accumulative strain grown at vinyl house (mean 18°C) in winter did not show anthocyanin color (purple) . However, this strain cultivated under outdoor condition (mean 10°C) accumulated anthocyanin pigment in plant tissues. These results suggest that the accumulation correlates closely to low air temperature. Both strains grown in nursery green had rhizoctonia brown patch disease in summer of 1998 and 1999. However, the extent of disease injury was larger in anthocyanin non-accumulative strain than that of anthocyanin accumulative strain. Anthocyanin non-accumulative strain contained more TNC, sugar and lesser starch than anthocyanin accumulative strain in winter. While, the latter strain had more starch in summer than the former. These results suggest that growth of penncross cultivar in winter is supported by anthocyanin non-accumulative strain, and that the heat tolerance and disease resistance in summer are supported by anthocyanin accumulative strain.