Relationship between high-molecular mass intestinal alkaline phosphatase detected in blood group B and O secretors and intestinal variant alkaline phosphatase
- Resource Type
- Journal Article
- Authors
- Makoto Matsushita; Tsugikazu Komoda; Tsutomu Irino
- Source
- SEIBUTSU BUTSURI KAGAKU. 2000, 44(2):135
- Subject
- IAP variant
PAGE
agarose gel electrophoresis
high-molecular mass IAP (HIAP)
- Language
- English
- ISSN
- 0031-9082
1349-9785
We have studied a relationship between high-molecular mass intestinal alkaline phosphatase (HIAP) found in blood group B and O secretors on 6.0% polyacrylamide disc gel electrophoresis in the presence of 1.0% TritonX-100 and the intestinal variant alkaline phosphatase (IAP-variant) on agarose gel electrophoretic method. The serum of 53 healthy subjects was tested by above two electrophoretic methods. The HIAP and IAP-variant were detected in the same subjects, and the levels of their activity were higher in serum of blood group B and O secretors. In addition, the HIAP and IAP-variant can be normalized to usual IAP by brief treatment with subtilisin or papain on respective methods. These results suggest that HIAP and TAP-variant are the same IAP isoform.