We provide lectures and questionnaire surveys on folic acid for registered dietitian course students and nursing course students. They may have the opportunity to be involved in future patient health care and provide important advice on the involvement of folic acid in neural tube obstruction (mainly spina bifida and anencephaly). The food intake frequency survey and the dietary habit questionnaire were administered to 980 female students aged 18 to 22 (registered dietitian course students: 730, nursing course students: 250). Among registered dietitian course students (N = 258), the intake of folic acid by students who did not omit meals was significantly (p < 0.05) higher than that by those who answered that they omit meals. Moreover, it was significantly (p < 0.05) higher for students whoresponded that their dietary status was “Good” than for those who answered “Problematic”. Among nursing course students (N = 52), there was no difference in the presence of the omission of meals or the intake of folic acid. In addition, it was significantly (p < 0.05) lower for students who answered “Problematic” for their dietary status than for those who answered “Neither good nor problematic”. Registered nutrition course students promoted the intake of folic acid as their dietary habits and dietary consciousness improved. However, there was no relationship among dietary habits, consciousness, and folic acid intake in nursing course students.