Suicide is a serious social problem, especially in Japan. The Showa University Style Psychiatric Health Checkup has been carried out for Japanese working peoples at Small and Medium-sized Enterprises (JWP-SME) who received insufficient mental care to prevent suicide since October 2008. The purpose of this study was to examine the psychiatric morbidity rate and relation risk of suicide-related behavior and psychiatric disorders, such as mood and anxiety disorder using checkup data. JWP-SME (N=1,411 M/F: 1,047/364 average age: 40.1±10.3 years old) were recruited between October and November 2014. We used Mini-International Neuropsychiatric Interview (M.I.N.I.), the Center for Epidemiological Studies-Depression (CES-D), Bipolar Spectrum Diagnostic Scale (BSDS), and Liebowitz Social Anxiety Scale-Japanese version and Sheehan Disability Scale (SDISS). We defined psychiatric disorders as psychiatric disorders Screening (Sc) according to our method. The results from M.I.N.I.data indicated that JWP-SME have mood and anxiety disorders and the risk of suicide-related behaviors. Those with a higher risk of suicide-related behaviors JWP-SME had higher scores of CES-D, BSDS, LSAS, and SDISS. In multivariate analyses, dysthymia Sc (adjusted odds ratio [aOR] 3.72), panic disorder Sc (aOR 2.93), bipolar disorder Sc (aOR 2.73), major depressive disorder Sc (aOR 2.66), post-traumatic stress disorder Sc (aOR 2.18), obsessive-compulsive disorder Sc (aOR 1.98), female (aOR 1.77), and young age (aOR 0.96) were all independently associated with the risk of suicide related behaviors. These results indicate that Japanese psychiatrists should evaluate mood and anxiety disorders for JWP-SME's prevention of suicide.