Immunostimulatory Effects of Cycloartane-Type Triterpene Glycosides from Astragalus Species
- Resource Type
- Journal Article
- Authors
- David S. PASCO; Erdal BEDIR; Ihsan CALIS; Ikhlas A. KHAN; Nirmal PUGH
- Source
- Biological and Pharmaceutical Bulletin. 2000, 23(7):834
- Subject
- Astragalus
THP-1
astragaloside I
immunostimulatory
macrophage
nuclear factor kappa B (NF-κB)
- Language
- English
- ISSN
- 0918-6158
1347-5215
In the course of our research on the oligoglycosidic constitutents of Turkish Astragalus species, we have isolated a number of cycloartane-type triterpene glycosides. The current study examines the immunostimulatory effects of nineteen of these cycloartane-type compounds using a transcription-based bioassay for Nuclear Factor kappa B (NF-κB) activation in a human macrophage/monocyte cell line, THP-1. All compounds were inactive at 100 μg/ml except astragaloside I which increased NF-κB directed luciferase expression to levels about 65% as compared with maximal stimulation by E. coli lipopolysaccharide (LPS) at 10 μg/ml. None of the compounds were active at low dosage levels (0.1 μg/ml) in combination with 50 ng/ml LPS. Astragaloside I also increased mRNA expression of the inflammatory cytokines interleukin-1β(IL-1β) and tumor necrosis factor-α (TNF-α) as measured using reverse transcriptase-polymerase chain reaction (RT)-PCR. Based on these results it is clear that certain structural features are required for immunostimulation of cycloartane-type triterpene glycosides.