The laser-induced fluorescence method (LIF) has been attracted as an available diagnostic tool for quantitative measurements in wind tunnel testing. Acetone is an ideal tracer material for the LIF method because of low toxicity and its strong fluorescence intensity. Although many studies on the acetone LIF method have been reported, most of these studies are limited to high temperature conditions. In the present study, we investigate the acetone LIF characteristics in low temperature conditions. The 4th harmonic of the Nd:YAG laser (266nm) is used to excite the acetone molecule and the resulting fluorescence intensity is detected by the photomultiplier tube. Nitrogen is selected as a buffer gas. The experimental result reveals that the LIF intensity was almost constant in the present temperature range of 270-300K.