Results: The number of times intravenous antibiotics were administered in 2016, 2017, 2018, and 2019 were 279, 380, 428, and 350, respectively. The AUD values decreased significantly over time from 3.12 in 2016 to 0.93 in 2019 (p<0.05). A significant difference was reported in the proportion of Pseudomonas aeruginosa isolates susceptible to meropenem in 2016 (50%) and after 2017 (>80%), following the introduction of the pediatric ASP (p<0.01). Although the AUD of vancomycin increased with an increase in the number of patients admitted to the PICU, no vancomycin-resistant strains were detected.