Accumbal GABAA and GABAB receptors each inhibit acetylcholine efflux without affecting dopamine efflux in the nucleus accumbens of freely moving rats / 無麻酔非拘束ラットの側坐核のGABAAおよびGABAB受容体は同部位のドパミン放出に影響を与えずにアセチルコリン放出を抑制する
- Resource Type
- Journal Article
- Authors
- Manabu Ishikawa; Masataka Kimura; Tadashi Saigusa; Yuri Aono; Yuriko Watanabe; 三枝 禎; 木村 全孝; 渡邉 由梨子; 石川 学; 青野 悠里
- Source
- Proceedings for Annual Meeting of The Japanese Pharmacological Society. 2019, :1-002
- Subject
- Language
- Japanese
- ISSN
- 2435-4953
We analyzed the roles of GABAA and GABAB receptors (-Rs) in regulating acetylcholine (ACh) efflux in the nucleus accumbens (NAc) of freely moving rats using in vivo microdialysis. The effects of GABA-R ligands on accumbal dopamine (DA) efflux were also analyzed as accumbal cholinergic and dopaminergic neurons could mutually interact. Drugs used were infused directly into NAc. The GABAA-R agonist muscimol (30 pmol) and the GABAB-R agonist baclofen (300 pmol) each reduced accumbal ACh. The GABAA-R antagonist bicuculline (60 pmol) counteracted the muscimol (30 pmol)-induced decrease in ACh and the GABAB-R antagonist saclofen (12 nmol) counteracted the baclofen (300 pmol)-induced decrease in ACh. Each of muscimol (30 pmol), baclofen (300 pmol), bicuculline (60 pmol) and saclofen (12 nmol) did not alter baseline accumbal DA when given alone. Doses of compounds indicate total amount infused (mol) during 30-60 min infusions. These results show that GABAA and GABAB-Rs exert inhibitory roles in the control of accumbal ACh efflux. This study provides in vivo evidence that GABAA and GABAB-Rs each reduce accumbal cholinergic activity without affecting accumbal dopaminergic activity.