Double Lesions are Produced in DNA Oligomer by Ionizing Radiation and by Metal-Catalyzed H2 O2 Reactions
- Resource Type
- research-article
- Authors
- Patrzyc, Helen B.; Dawidzik, Jean B.; Budzinski, Edwin E.; Iijima, Herbert; Box, Harold C.
- Source
- Radiation Research, 2001 Apr 01. 155(4), 634-636.
- Subject
- Lesions
Reactive oxygen species
Free radicals
Peroxides
Superoxides
Elution
Oligomers
Hydrogen
DNA damage
Ionizing radiation
- Language
- English
- ISSN
- 00337587
19385404
It was demonstrated previously that double lesions are produced in DNA by ionizing radiation. These double lesions consist of adjacent nucleotides each bearing a modified base. The goal of the present investigation was to determine whether Fenton chemistry can generate the same kind of lesions. DNA oligomers were exposed to metal-catalyzed H2 O2 reactions, and the products were characterized by chromatography and by mass spectrometry. Double lesions are produced by this treatment in which deoxyguanosine is oxidized to 8-oxo-7,8-dihydrodeoxyguanosine and an adjacent pyrimidine nucleoside is degraded to a formamido remnant.