目的: 探讨松果提取物作为生物活性成分和化妆品成分时的性质和潜在的商业价值。方法: 用热水(WE),70% 乙醇(EE)和超临界流体萃取(SFE)提取松果,为了确定每种提取方法获得的提取物的植物化学成分,通过 GC/MS进行了分析,并通过总多酚含量和2,2-二苯基-1-吡啶并肼基(DPPH)自由基清除能力来测定抗氧化活 性。另外,比较和分析了抗微生物和抗炎作用,并将每种提取物添加到乳液配方中以确认稳定性。结果: 测量 总多酚含量的结果:WE为180.75±1.83 mg/g,EE为252.69±0.34 mg/g,SFE为270.10±0.97 mg/g。通过 DPPH法测定抗氧化效果时,即使在低浓度(0.125%)下,通过SFE获得的提取物也显示出高抗氧化能力。 测量松果提取物的抗菌效果的结果是,SEF提取物在SA中显示出12.1±1.04 mm的透明区域,在SE中显示出 11.8±0.45 mm的透明区域,显示出比其他WE和EE提取物更高的抗菌效果。细胞毒性评估的结果,在所有提取 物中均观察到80%以上的细胞生存率。在抗炎实验中,证实了与LPS治疗组相比,一氧化氮的表达显着降低。 制备含有松果提取物的乳液并观察到稳定性的结果,证实了仅含有SFE提取物的乳液在pH和高温(40℃)下是 稳定的。结论: 这些研究结果表明,SFE提取物是一种可能的药妆材料,具有强大的皮肤再生和抗炎特性。
Purpose: This study examines the properties and potential commercial value of the pine cones extract when used as bioactive and cosmetics ingredients. Methods: Pine cones were extracted with hot water (WE), 70% ethanol (EE), and supercritical fluid extraction (SFE) to examine their antioxidant properties on the basis of their total polyphenol content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and gas chromatography-mass spectrometry (GC-MS) analysis. The antibacterial properties of the pine cone extract were also studied on 2 types of bacteria. In addition, this study examined the nitric oxide (NO) inhibition effect using RAW 264.7 cells, in which the inflammatory reaction was induced by lipopolysaccharides (LPS), and cell migration activity in the HeCaT cells. Results: The extract obtained with SFE exhibited high antioxidative ability even at low concentration (0.125%). The antimicrobial activity of the of SFE pine cone extract was 12.1±1.04 mm for Staphylococcus aureus (S. aureus ) and 11.8±0.45 mm for Staphylococcus epidermidis (S. epidermidis ). Cytotoxicity evaluation showed cell viability above 80% in all extracts. During the antiinflammatory test, the nitric oxide expression level was low, and it was confirmed by the antiinflammatory effect. The results of the stability test showed that emulsions containing SFE extract were very stable. The pH and high temperature (40℃) of each emulsion containing SFE extract did not considerably change for 30 days. Conclusion: These findings suggest that SFE extract is a possible cosmeceutical material with potent skin regeneration and antiinflammatory properties.