This study focused on the Gaya mural tomb located in Goa-ri, Goryeong-gun, North Gyeongsang Province, Republic of Korea. Environmental factors such as ambient temperature, relative humidity and surface temperature, and microbial distribution on mural tombs were monitored for one year to gather data on the conservation environment of the mural tombs. The average internal temperature difference with reference to the ambient outer temperature was observed to be 11.7℃ for the monitoring period and the internal temperature of the tomb was found to change periodically everyone or two months in response to the outer temperature. The highest temperature was observed in September and the lowest in March. The relative humidity in the mural tomb remained constant at 100%. Between December and April, condensation occurred on the ceiling of the main room of the tomb, where the murals are located. On the ceiling of the aisle, the condensation occurred throughout the year. The inside and surface wall were isolated from microorganisms, which could grow when a suitable growth environment suitable is established. Based on microbial growth temperature conditions, risk periods of microbial hazards were established, where in the period from August to October was identified as the most dangerous.