This study aims to understand the effects of nursing college students’ anxiety about clinical practice and stress from it during the practice on their nursing professionalism during COVID-19 and be used as baseline data to promote this professionalism. The target is 187 juniors and seniors in nursing college located in Chungnam, Chungbuk, and Gyeongbuk with experience in the clinical practice, and the survey was conducted from September to November 2021. The data was analyzed by t-test, ANOVA, Pearson correlation coefficient, and multiple regression using SPSS 22.0. Nursing professionalism was significantly correlated with clinical practice stress(r=-.151, p=.039). In terms of the factors that influenced the nursing professionalism, the coefficient of determination of satisfaction in major was 5.2%(β=-.24, p=.001), and that of stress from the clinical practice was 3.0%(β=-.17, p=.015), which explains a total of 8.2%. Major satisfaction was the most influential factor predicting greater nursing professionalism. The results of this study demonstrate the necessity to increase nursing students’ satisfaction in major and reduce their stress from clinical practice in order to improve the nursing professionalism during COVID-19.