目的: 堪察加景天(Sedum kamtschaticum Fisch)(SK) 是一种原产于韩国的植物,被研究作为天然防腐剂和 抗衰老化妆品成分。方法: 将SK的茎和根分离;用70%乙醇回流提取;并用正己烷、氯仿、乙酸乙酯、正丁醇 和水分级。评估了对每个部位具有最高抗氧化效果的乙酸乙酯组分的抗衰老作用,包括抗糖化活性以及弹性蛋 白酶和胶原酶抑制活性。使用纸盘扩散法和激发试验评估了它们对七种微生物的抗菌活性。此外,还使用弗朗 茨扩散池方法评估了乳液配方的稳定性和皮肤渗透性。结果: 抗氧化效果最好的SK乙酸乙酯组分比阳性对照表 现出更好的抗糖化和胶原酶抑制活性,并且对大肠杆菌和金黄色葡萄球菌具有优异的抗菌作用。含有0.05%和 0.1% SK茎提取物的乳液制剂表现出协同防腐作用。SK茎提取物具有渗透人造膜和人体尸体皮肤的能力。结论: SK提取物可作为抗衰老、抗菌化妆品原料。
Purpose: Sedum kamtschaticum Fisch. (SK), a plant native to Korea, was studied as a natural preservative and antiaging cosmetic ingredient. Methods: The stems and roots of SK were separated; extracted through reflux with 70% ethanol; and fractionated with n-hexane, chloroform, ethyl acetate, n-butyl alcohol, and water. The ethyl acetate fractions were evaluated for their antiaging effects, including antiglycation activity and elastase and collagenase inhibitory activity. Their antimicrobial activity on seven microorganisms was evaluated using the paper disk diffusion method and challenge test. In addition, the stability of lotion formulations and skin penetration were assessed using the Franz diffusion cell method. Results: The ethyl acetate fractions of SK with the best antioxidant effects exhibited better antiglycation and collagenase-inhibitory activity than the positive control as well as excellent antibacterial effects against Escherichia coli and Staphylococcus aureus. Emulsion formulations containing 0.05% and 0.1% SK stem extract exhibited synergistic preservative effects. The SK stem extract displayed the ability to penetrate into an artificial membrane and human cadaver skin. Conclusion: SK extracts can be used as antiaging and antimicrobial cosmetic materials.