Today's electromagnetic countermeasures environment is complicated, and satellite navigation signals are relatively easy to be interfered by electromagnetic interference. At the same time, because the navigation signals are based on satellite communication, their signals are weak when they reach the receiver and are easily interrupted by interference. In order to achieve anti-interference and precise positioning in a complex electromagnetic environment, it is necessary to know the direction of the interference signal. Aiming at this problem, this paper has carried out the research on the direction of arrival estimation of the interference signal. By analyzing different antenna array models, this paper designs a six-element uniform circular array antenna, based on the MUSIC algorithm, and has made certain improvements. In this paper, the angle of the suppressive interference signal is detected, and the influence of different factors on the estimation is analyzed. Through experimental simulation, it is verified that the method proposed in this paper can accurately estimate the direction of the interference signal and achieve good results.