Highly-Sensitive SPR Urea Biosensor Based on Urease Immobilized in Metal-Organic Zeolite Framework
- Resource Type
- Conference
- Authors
- Cheng, Liangliang; Zheng, Wanlu; Li, Like; Zhang, Ya-nan
- Source
- 2021 19th International Conference on Optical Communications and Networks (ICOCN) Optical Communications and Networks (ICOCN), 2021 19th International Conference on. :1-3 Aug, 2021
- Subject
- Communication, Networking and Broadcast Technologies
Components, Circuits, Devices and Systems
Fields, Waves and Electromagnetics
Photonics and Electrooptics
Signal Processing and Analysis
Optical fibers
Optical fiber sensors
Gold
Sensitivity
Refractive index
Optical fiber networks
Sensors
highly-sensitive
Urease
ZIF-8
SPR
Urea
- Language
A highly-sensitive multi-mode thin-core multi-mode (MTM) surface plasmon resonance (SPR) structure is proposed and applied in urea sensing. The thin-core fiber is sputtered with gold film to excite SPR, and then the probe is coated with enzyme coating that is formed by embedding urease in zeolitic imidazolate framework (ZIF -8/Urease) to realize a highly specific urea biosensor. The bind of urea and urease in the zeolitic imidazolate framework will cause the change of refractive index of the SPR probe, which will then induce the wavelength shift of SPR resonance spectrum. Experimental results demonstrated that the resonance wavelength has a good linear relationship with the urea concentration in the range of 1–10 mM, and the sensitivity can reach to 4 nm/mM.