A Digital Signal Processing-Assisted Microfluidic PCB for On-Chip Fluorescence Detection
- Resource Type
- Periodical
- Authors
- Babikian, S.; Li, G.P.; Bachman, M.
- Source
- IEEE Transactions on Components, Packaging and Manufacturing Technology IEEE Trans. Compon., Packag. Manufact. Technol. Components, Packaging and Manufacturing Technology, IEEE Transactions on. 7(6):846-854 Jun, 2017
- Subject
- Components, Circuits, Devices and Systems
Engineered Materials, Dielectrics and Plasmas
Optical sensors
Optical imaging
Shape
Integrated optics
Optical signal processing
Signal to noise ratio
Digital signal processing (DSP)
fluorescence detection
heterogeneous integration
isotachophoresis (ITP)
lab-on-chip
microfluidics
printed circuit board (PCB)
- Language
- ISSN
- 2156-3950
2156-3985
We report the use of digital signal processing (DSP) strategies to realize high-sensitivity on-chip fluorescence detection in microfluidic-opto-electronic printed circuit board (PCB) devices without requiring bulky high-quality optical components and filters. An isotachophoresis (ITP) assay was studied in a microfluidic PCB with an embedded standard complementary metal oxide semiconductor sensor array demonstrating a detection sensitivity of 10-nm initial concentration for fluorescein. The amplified ITP signal is processed with the DSP algorithms allowing the fluorescence signal extracted from noisy sensor output to achieve high signal-to-noise ratio. The DSP-assisted fluorescence detection system utilizes off-the-shelf, consumer-grade electronics and lensless imaging to achieve high-level but also scalable integration on microfluidic PCBs.