At present, IPv6 faces the problem of compatibility with IPv4 in large-scale applications. The transition technologies between IPv6 and IPv4 can be divided into three kinds: dual protocol stack technology, tunnel technology encapsulated by IP data gram and transparent conversion technology. Based on the research on the transition technology of IPv4 and IPv6, taking the enterprise network application as the object, through the experimental simulation implementation mode, compare the relevant configuration schemes, simulate the protocol transition technology in the scheme construction, allocate IPv6 addresses on the line, discuss the security technology of IPv6, and realize the transition of IPv4 and IPv6 under the enterprise network architecture.