Having borrowed ideas from the principle of ecological niche, this paper presents the concept of “ecological niche” of enterprise. In this paper, ecological niche theory is used to discuss the enterprise ecological niche and the effect on enterprises’ existence, developing. Abided by the following principles during the formation of their ecological niches: the principle of adaptation, the principle of competition, the principle of reclamation and the principle of balance, which refers to three further competitive strategies of enterprise: the strategy of dislocation business, the strategy of ecological niche continuation and the strategy of dummy ecological niche.