Digital Twin (DT) technology, which extract test scenarios from real-world driving data, is considered as an effective solution for ICVs (Intelligent and connect vehicle) on-road test. However, due to the time-varying feature of environment, validity of current ICV oriented DT test scheme is hardly to verify. As a traditional test issue, In-chamber test is considered as an effective approach for vehicles’ regulation mandatory tests, such as EMC test. To promote standardization progress of ICV pre-market test, in this paper, an in-chamber C-V2X application test scheme is proposed, while corresponding test procedure is given. Moreover, two key technologies, test case design and GNSS control, are discussed. A cross validation progress is done to verify the validity of proposed scheme, and the effectiveness of test parameter setting, which is extracted from on-road acquisition data.